Uveitis

Model:

Endotoxin induced uveitis

  • An intravitreal (i.v.t.) injection of bacterial endotoxin (t=0) stimulates an influx of inflammatory cells, predominantly neutrophils, into the anterior uvea section of the eye.
  • This model is sensitive to pre-treatment with steroid, both by topical ( t= -30 min, 1hr, 2hr, 4hr) and intravitreal dosing (t=-1hr).

 

Background:

  • Uveitis, or intraocular inflammatory disease, is a damaging ocular condition which can cause cataracts, glaucoma, macular oedema, and eventually blindness.
  • It is estimated that the incidence of visual loss due to uveitis is on a par with that of diabetes, and is the cause of 10-15% of all total blindness in the United States.
  • Current therapies for uveitis, and many other chronic inflammatory disorders such as asthma, are steroids, immunosuppressive agents and non-steroidal anti-inflammatories (NSAIDS).
  • These drugs do not completely control the disease processes and are not well tolerated due to the development of multiple adverse effects, and length of duration of treatment.

Animal:

  • Rat

Inducer:

  • LPS

 Study Duration:

  • Approximately 3-4 weeks

 Parameter Measures:

  • Total cell counts in the aqueous humor (fluid between the lens and the cornea of the eye)
  • Cytokine analysis of the anterior/posterior eye homogenate
  • Histological assessment of cellular infiltration

 Cell Data

Uveitis - cell data ivt dex graph

Intravitreal (I.v.t.) administration of LPS induces a significant increase in the number of total inflammatory cells recruited into the aqueous humor, which is reduced following single dose of i.v.t. dexamethasone.

 Cytokine Data

 

Uveitis - cytokine graph MCP-1 (ivt) Uveitis - cytokine graph IL-B (ivt)

Intravitreal (I.v.t.) administration of LPS induces an increase in IL-1B and MCP-1 levels in the anterior uvea, which is reduced following single dose of i.v.t. administered dexamethasone.

Uveitis - cytokine graph MCP-1 (top)Uveitis - cytokine graph IL-B (top)

Intravitreal (I.v.t.) administration of LPS induces an increase in IL-1B and MCP-1 levels in the anterior uvea, which is reduced following multiple doses of topical dexamethasone.

 

# compared to control, * compared to LPS

 

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