Ulcerative Colitis/Intestinal Fibrosis
- Administration of either dextran sodium sulfate (DSS) via drinking water or, 2,4,6- trinitrobenzenesulfonic acid (TNBS) dosed intra-rectally, can cause varying degrees of colon inflammation depending on study duration.
- Disturbances of intestinal homeostasis result in chronic inflammatory disorders of the intestine that are also referred to as inflammatory bowel disease (IBD). Two major forms are ulcerative colitis (UC) and Crohn’s disease (CD).
- The aetiology of the disease is still not completely understood. Contributors to the initiation and progression of the disease are generally accepted to be a complex interplay between genetic, environmental and immunological factors.
- Symptoms of patients suffering from IBD are abdominal cramping, diarrhoea, rectal bleeding and weight loss.
- Due to the complexity of the disease in humans, the development of an animal model which can display such heterogeneous complications has proven to be difficult. Rather, animal models have demonstrated to be valuable tools in understanding various disease aspects.
- Mouse and Rat
- Disease Activity Index (DAI) : scoring weight loss, stool consistency and bleeding
- Wallace scoring method (inflammation grading of colonic damage)
- Myeloperoxidase (MPO) assay
- Cytokine analysis
Intrarectal administration of a single dose of PBS, 50% ethanol or 100mpk TNBS l (I.v.t.) induces a significant increase in average colonic damage scores and myeloperoxidase (MPO) activity measured in colon sections in mice after 48 hours. *MPO: peroxidase enzyme most abundantly expressed in neutrophil granulocytes, indicative of neutrophil activity.
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