Acute Lung Injury

Model:

  • Airway exposure to bacterial endotoxin produces a robust localised neutrophilic inflammation.

Background:

  • Neutrophilic inflammation is a key contributor in the pathogenesis of diseases such as COPD and Acute Lung Injury, making this a useful model for screening compounds targeting either condition.

Animal:

  • Mice and Rats

Inducer:

  • Lipopolysaccharide (LPS)

Dosing routes:

  • P.O., I.P., I.T. S.C., I.N. and I.V.

Study Duration:

  • Approximately 1-2 weeks

Parameter Measures:

  • BALF cellular infiltration
  • Cytokines in the Blood/BALF
  • Histology
  • Organ or fluid collection for external analysis e.g. PK analysis
  • Western Blot
  • PCR

Mouse BALF Cell Data

Acute lung injury - total neutrophils

Mouse BALF Cytokine Data

Acute lung injury - IL-18              Acute lung injury - IL-6

Rat Data

Acute lung injury - rat total cells   Acute lung injury - rat total neutrophils

*compared to sham

Acute lung injury - rat total cells time courseAcute lung injury - rat total neutrophils time course

*compared to corresponding untreated group

 

Downloadable Files