Asthma exacerbation (influenza)

Model:

  • Influenza infection following acute allergen sensitisation to house dust mite produces a localised inflammation, characterised by an influx of eosinophils, neutrophils, and mononuclear cells, which is steroid insensitive.
  • This model demonstrates exacerbation of both Th1 and Th2 cytokines, and inflammatory cell infiltrates of the BAL fluid, as well as airway hyperresponsiveness.

 Background:

  • Influenza A (IVA) is a common respiratory infection often resulting in acute exacerbations amongst asthmatics. Drug treatment e.g. steroids for exacerbations are generally less effective resulting in increased hospitalization and mortality amongst asthmatics, making this a highly relevant and useful model for screening compounds targeting this condition.

Animal:

  • Mouse

Inducer:

  • House dust mite and mouse adapted influenza A virus

Dosing routes:

  • P.O., I.P., I.T. S.C., I.N. and I.V.

Study Duration:

  • Approximately 4-6 weeks

Parameter Measures:

  • BALF cellular infiltration
  • Cytokines in the Blood/BALF/Lung
  • Histology
  • Viral Titre
  • Lung function
  • Western Blot
  • PCR

Viral titre dose-response

N.B. UV refers to ultra-violet inactivated IVA

Asthma exacerbation-viral titre dose-response total cells                       Asthma exacerbation-viral titre dose-response total eosinophils

 

Asthma exacerbation-viral titre dose-response total neutrophils                       Asthma exacerbation-viral titre dose-response total macrophages

* compared to respective saline group

 

Time course data

Asthma exacerbation-time course total eosinophils                    Asthma exacerbation-time course total cells

N.B. Viral Titre 1 x 103 PFU

 

Steroid data (Dexamethasone)

Asthma exacerbation - steroid total cells

 

Asthma exacerbation - steroid total eosinophils

# compared to naïve

* compared to HDM/HDM + UV IVA

 

Airway hyper-responsiveness 

Asthma exacerbation - RI (AUC)

N.B. Viral Titre 1 x 104 PFU

 

Downloadable Files